KMID : 0388220070140020112
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Journal of the Korean Rheumatism Association 2007 Volume.14 No. 2 p.112 ~ p.117
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Upregulation of Macrophage Migration Inhibitory Factor (MIF) Production from Peripheral Blood Mononuclear Cells (PBMCs) Stimulated by Tumor Necrosis Factor (TNF)-¥á in Patients with Behcet¡¯s Syndrome
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Kwok Seung-Ki
Cho Mi-La Kim Ho-Youn Park Mi-Kyung Ju Ji-Hyeon Park Sung-Hwan Seo Soo-Hong Yoon Chong-Hyun
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Abstract
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Objective: To investigate the effect of tumor necrosis factor (TNF)-¥á on the production of macrophage migration inhibitory factor (MIF), which might have important roles in the immune mediated inflammatory response of Behcet¡¯s syndrome.
Methods: Sixty two patients with Behcet¡¯s syndrome and thirty healthy controls were included in this study. The concentrations of TNF-¥á in sera were determined by enzyme-linked immunosorbent assay (ELISA). Peripheral blood mononuclear cells (PBMCs) from eleven patients with Behcet¡¯s syndrome were cultured for 48 hours with various concentration of TNF-¥á. The concentrations of MIF in sera and culture supernatants were determined by ELISA.
Results: Serum levels of TNF-¥á were significantly higher in patients with Behcet¡¯s syndrome than in healthy controls. TNF-¥á dose-dependently increased MIF production from PBMCs in patients with Behcet¡¯s syndrome. Serum levels of TNF-¥á tended to correlate with serum levels of MIF, although did not reach statistical significance.
Conclusion: Upregulation of MIF production by increased levels of TNF-¥á in patients with Behcet¡¯s syndrome might be related to the pathogenesis of Behcet¡¯s syndrome.
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KEYWORD
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Behcet¡¯s syndrome, TNF-¥á, MIF
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